238000005352 clarification Methods 0.000 claims description 15.235000011130 ammonium sulphate Nutrition 0.000 claims description 15.229910052921 ammonium sulfate Inorganic materials 0.000 claims description 15.BFNBIHQBYMNNAN-UHFFFAOYSA-N Ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims description 15.KCXVZYZYPLLWCC-UHFFFAOYSA-N edta Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 claims description 16.238000005571 anion exchange chromatography Methods 0.000 claims description 20.238000005336 cracking Methods 0.000 claims abstract description 5.238000004255 ion exchange chromatography Methods 0.000 claims abstract description 6.239000007788 liquid Substances 0.000 claims abstract description 8.238000000108 ultra-filtration Methods 0.000 claims abstract description 12.238000001914 filtration Methods 0.000 claims abstract description 15.238000001042 affinity chromatography Methods 0.000 claims abstract description 18.238000000746 purification Methods 0.000 title claims abstract description 29.229920003013 deoxyribonucleic acid Polymers 0.000 title claims abstract description 71.Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.) Filing date Publication date Application filed by Heng Ruiyuan Zheng Guangzhou Biotechnology Co ltd, Hengrui Yuanzheng Shenzhen Biological Technology Co ltd, Henry Is Source Of Biological Science And Technology Co ltd Shanghai filed Critical Heng Ruiyuan Zheng Guangzhou Biotechnology Co ltd Priority to CN202010129401.2A priority Critical patent/CN111304193A/en Publication of CN111304193A publication Critical patent/CN111304193A/en Status Pending legal-status Critical Current Links Original Assignee Heng Ruiyuan Zheng Guangzhou Biotechnology Co ltd Hengrui Yuanzheng Shenzhen Biological Technology Co ltd Henry Is Source Of Biological Science And Technology Co ltd Shanghai Priority date (The priority date is an assumption and is not a legal conclusion. Henry Is Source Of Biological Science And Technology Co ltd Shanghai ![]() Hengrui Yuanzheng Shenzhen Biological Technology Co ltd Heng Ruiyuan Zheng Guangzhou Biotechnology Co ltd Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.) ![]() Inventor 石先灯 李进 吴健华 Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.) Pending Application number CN202010129401.2A Other languages Chinese ( zh) Google Patents Method for large-scale rapid purification of plasmid DNAĭownload PDF Info Publication number CN111304193A CN111304193A CN202010129401.2A CN202010129401A CN111304193A CN 111304193 A CN111304193 A CN 111304193A CN 202010129401 A CN202010129401 A CN 202010129401A CN 111304193 A CN111304193 A CN 111304193A Authority CN China Prior art keywords plasmid dna concentration column purification anion exchange Prior art date Legal status (The legal status is an assumption and is not a legal conclusion. Google Patents CN111304193A - Method for large-scale rapid purification of plasmid DNA CN111304193A - Method for large-scale rapid purification of plasmid DNA
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